ANCA-Associated Vasculitis Profile VASCAB

Method(s) Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay
Specimen Required
Collect One 5 mL gold (SST) top tube.
Transport 1 mL serum at 2-8°C (Min: 0.5 mL)
Remarks ORDERING RECOMMENDATION: Preferred reflex panel for the workup of suspected vasculitis. For patients with a history of vasculitis, refer to Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer and MPO/PR-3 Antibodies.
Stability After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Unacceptable Conditions CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
Schedule Mon-Sat
Billing Code 5011423
CPTCode 83516 MPO; 83516 PR-3; 86255 ANCA; if reflexed, add 86256 ANCA titer
Notes Specimens are screened for ANCA, MPO and PR-3. If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
Preferred Specimen Collection Device(s)
Reference Interval
ComponentReference Interval
Anti-Neutrophil Cytoplasmic Antibody, IgG<1:20: Not significant
Myeloperoxidase AntibodyNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater
Serine Protease 3 AntibodyNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater

Neutrophil cytoplasmic antibodies (C-ANCA=granular cytoplasmic staining, P-ANCA=perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis. Approximately 90 percent of patients with a P-ANCA pattern by IFA have antibodies specific for myeloperoxidase (MPO). Approximately 85 percent of patients with a C-ANCA pattern by IFA have antibodies specific for serine proteinase 3 (PR-3).

No Appointment Necessary
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12 Patient Service Centers in Central New York.

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