Testosterone, Bioavailable by LC/MS/MS, Females and Children (Includes Total Testosterone, SHBG and Free Testosterone) TSTLCB

Method(s) Liquid Chromatography-Tandem Mass Spectrometry and Immunoassay
Synonym Testosterone Free and Weakly Bound by LC/MS/MS (includes SHBG), Females and Children
Components

Total Testosterone, SHBG, Free Testosterone and Bioavailable Testosterone

Specimen Required
Collect

One 5 mL gold (SST) top tube.

Transport

1 mL serum 2-8ºC.

Remarks

Specimen should be collected between 6 and 10 am.

Stability

Ambient: 24 hours; Refrigerated: 1 week (3 days in contact with separator gel); Frozen: storage is not recommended

Schedule Mon, Thurs; reported Tues, Fri
Billing Code 1010468
CPTCode 84403 Total Testosterone; 84270 SHBG
Notes

The test is suggested for women and children due to the improved sensitivity of LC/MS/MS analysis of testosterone.

The concentration of free testosterone is derived from a mathematical expression based on the constants for the binding of testosterone to sex hormone binding globulin and albumin.

Preferred Specimen Collection Device(s)
Reference Interval
Sex Hormone Binding Globulin
FemaleMale
20-46 years: 18-136 nmol/L
47 years and older: 17-125 nmol/L
13-90 nmol/L

 

Bioavailable Testosterone, LCMS
FemaleMale
1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-5.0 ng/dL
10-11 years: 0.4-9.6 ng/dL
12-13 years: 1.7-18.8 ng/dL
14-15 years: 3.0-22.6 ng/dL
16-17 years: 3.3-28.6 ng/dL
18-30 years: 2.2-20.6 ng/dL
31-40 years: 4.1-25.5 ng/dL
41-51 years: 2.8-16.5 ng/dL
52 years and older: 1.5-9.4 ng/dL
Postmenopausal: 1.5-9.4 ng/dL
Tanner Stage I: 0.3-5.5 ng/dL
Tanner Stage II: 1.2-15.0 ng/dL
Tanner Stage III: 3.8-28.0 ng/dL
Tanner Stage IV: 2.8-39.0 ng/dL
Tanner Stage V: 2.5-23.0 ng/dL
1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-2.8 ng/dL
10-11 years: 0.1-17.9 ng/dL
12-13 years: 1.4-288.0 ng/dL
14-15 years: 9.5-337.0 ng/dL
16-17 years: 35.0-509.0 ng/dL
18 years and older: 130-680 ng/dL
Tanner Stage I: 0.3-13.0 ng/dL
Tanner Stage II: 0.3-59.0 ng/dL
Tanner Stage III: 1.9-296.0 ng/dL
Tanner Stage IV: 40.0-485.0 ng/dL
Tanner Stage V: 124.0-596.0 ng/dL
No Appointment Necessary
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