Testosterone, Free and Total by Immunoassay, Adult Male TSTRF

Method(s) Chemiluminescent Immunoassay
Components Total Testosterone, SHBG, Free Testosterone
Specimen Required
Collect

One 5 mL gold (SST) top tube. Heparinized plasma also acceptable.

Transport

1 mL serum at 2-8°C.

Remarks

Specimen should be collected between 6 and 10 am.

For females and children under 14 years, select LCMS-based assays for required sensitivity.

Stability

Ambient: 8 hours; Refrigerated: 2 days; Frozen: 1 month

Schedule Daily
Billing Code 1010459
CPTCode 84403 Total testosterone, 84270 SHBG
Notes

The concentration of free testosterone is derived from a mathematical expression based on the constants for the binding of testosterone to sex hormone binding globulin and albumin.

Results of the testosterone component of this test may be falsely elevated in patients taking over-the-counter biotin supplements.

Results of the SHBG component of this test may be falsely decreased in patients taking over-the-counter biotin supplements.

Preferred Specimen Collection Device(s)
Reference Interval
 
ComponentReference Interval
Testosterone Free, Immunoassay


Male:
14-15 years: 3-138 pg/mL
16-17 years: 38-173 pg/mL
18 years and older: 47-244 pg/mL
Testosterone, Total, ImmunoassayAdult Male:
Less than 50 years: 123 - 814 ng/dL
Greater than or equal to 50 years: 87 - 780 ng/dL
Sex Hormone Binding GlobulinAdult Male:
Less than 50 years: 15 - 95 nmol/L
Greater than or equal to 50 years: 22-113 nmol/L
Testosterone, Percent FreeMale, adult:
1.6 - 2.9%
No Appointment Necessary
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12 Patient Service Centers in Central New York.

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