Parathyroid Hormone-Related Peptide by LC-MS/MS, Plasma PTHREP
|Method(s)||Quantitative High Performance Liquid Chromatography - Tandem Mass Spectrometry|
Protease Inhibitor tube.
1.5 mL plasma. (Min: 0.7 mL)
|Remarks||A butterfly collection set must be used. NOT RECOMMENDED: Filling collection tubes directly through a needle/tube-holder assembly increases the risk of chemical reflux back into the vein of the patient. Mix collection tube thoroughly. Separate the plasma from cells within 1 hour of collection by centrifugation.|
After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
|Unacceptable Conditions||Grossly hemolyzed specimens.|
|Schedule||Sun, Wed, Fri|
Amino (N)- and carboxy (C)-terminus PTHrP fragments, such as those produced by some patients with renal insufficiency, do not interfere with this assay.
|Preferred Specimen Collection Device(s)|