Mycobacterial (AFB) Culture and Smear AFBC
Respiratory specimens. Also acceptable: Body fluid, CSF, gastric aspirate, tissue, or urine.
Transport refrigerated. Specimen Preparation: Place each specimen in an individually sealed bag. Respiratory specimens: Transfer (for each collection) 5-10 mL to a sterile container (Min: 1 mL). Body Fluids or CSF: Transfer 5 mL to a sterile container (Min: 1 mL). Gastric Aspirates: Must be neutralized (pH7) with sodium carbonate if transport is delayed for more than four hours. Transfer 5-10 mL to a sterile container (Min: 1 mL). Tissue: Transfer to a sterile container and place on gauze moistened with sterile non-bacteriostatic saline to prevent drying (Min: Visible). Urine: Transfer at least 40 mL to a sterile container (Min: 10 mL).
Specimen source required. Patient Preparation: Recommended collection: Three sputum specimens at 8-24 hour intervals (24 hours when possible) and at least one first-morning specimen. An individual order must be submitted for each specimen.
Ambient: 24 hours; Refrigerated: 1 week, Frozen: 1 week
Dry material or material collected and transported on a wooden shaft swab. Acid Fast Stain: Stool, blood, bone marrow, grossly bloody specimens, CSF if less than 5 mL, or urine specimens if less than 40 mL.
|CPTCode||87116, 87206, 87015, 87556|
A direct specimen PCR test for M. tuberculosis complex in sputum is automatically performed on one of three sputum samples submitted for the detection of M. tuberculosis. It is also approved for sputum specimens submitted to remove patients from respiratory isolation. Cultures are routinely held for 8 weeks. A bacterial culture, fungal culture, and AFB culture will always be performed on bronchial specimens.
|Preferred Specimen Collection Device(s)|
No AFB isolated.