HIV-1, Genotyping HIVGEN
|Method(s)||Reverse Transcription Polymerase Chain Reaction/DNA Sequencing|
Two 5 mL lavender top (EDTA) or PPT tubes. Remove EDTA plasma from cells ASAP. Centrifuge PPT plasma ASAP.
|Transport||5 mL plasma, frozen. (Min: 2.5 mL).|
On cells: Ambient 6 hours. Removed from cells: Ambient 24 hours; Refrigerated: 5 days; Frozen: 4 months.
|Unacceptable Conditions||Serum, whole blood, or heparinized plasma. Specimens that are thawed or have been exposed to repeated freeze/thaw cycles.|
|Notes||Separate specimens must be submitted when multiple tests are ordered. Remove EDTA plasma from cells ASAP and freeze in a polypropylene tube at -20ºC for transport. Spin PPT ASAP and freeze at -20ºC or lower for transport. Viral load required.|
|Preferred Specimen Collection Device(s)|
See Laboratory Report.