Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer & MPO/PR3 Antibodies ANCAB

Method(s) Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay
Components ANCA; if positive reflexed to ANCA titer, MPO and PR3 ORDERING RECOMMENDATION: Preferred panel for evaluating and managing individuals with a known diagnosis of vasculitis. For suspected vasculitis workup, see Vasculitis Panel.
Specimen Required
Collect One 5 mL gold (SST) top tube.
Transport 1 mL serum at 2-8ºC. (Min: 0.5 mL) Separate serum from cells within 2 hours of collection.
Remarks Ordering recommendation: Preferred panel for evaluating and managing individuals with a known diagnosis of vasculitis.
Stability After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles).
Unacceptable Conditions Plasma, CSF, urine or body fluids. Severely lipemic, contaminated, or hemolyzed specimens.
Schedule Mon-Sat
Billing Code 5011116
CPTCode 86255; if reflexed add 86256; 83516 x 2
Notes Specimens are screened by IFA on ethanol-fixed neutrophils, formalin-fixed neutrophils, and HEp-2 slides that allow differentiation of C- and P-ANCA patterns. If screen is positive, then titer and MPO/PR3 antibodies will be added to aid in antibody determination. Additional charges apply.
Preferred Specimen Collection Device(s)
Reference Interval
ComponentsReference Interval
Anti-Neutrophil Cytoplasmic Antibody, IgG< 1:20: Not significant
Myeloperoxidase Antibody if reflexedNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater
Serine Protease 3 Antibody if reflexedNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater

Neutrophil cytoplasmic antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis. Approximately 90% of patients with a P-ANCA pattern by IFA have antibodies specific for myeloperoxidase (MPO). Approximately 85% of patients with a C-ANCA pattern by IFA have antibodies specific for serine proteinase 3 (PR-3).

No Appointment Necessary
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12 Patient Service Centers in Central New York.

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