Tissue Transglutaminase Antibody, IgA with Reflex to Endomysial Antibody, IgA Titer EDAB
|Method(s)||Enzyme Immunoassay/Indirect Fluorescent Antibody|
|Collect||One 5 mL gold (SST) top tube.|
1 mL serum at 2-8°C.
|Stability||After separation from cells: Ambient: 8 hours; Refrigerated: 7 days; Frozen: 1 year (avoid freeze/thaw cycles)|
Plasma. Contaminated, heat-inactivated or severely hemolyzed samples.
|CPTCode||83516 tTG IgA; if reflexed, add 86256 EMA titer|
|Notes||IgA class endomysial antibodies are seen in gluten-sensitive enteropathies. Approximately 70% of patients with dermatitis herpetiformis and almost 100% of patients with active celiac disease will demonstrate IgA antibodies. These antibodies generally decrease or become negative in patients on gluten-free diets and will usually reappear upon gluten challenge. All positives are titered to endpoint. If tTG IgA is 20 units or greater, then EMA titer will be added.|
|Preferred Specimen Collection Device(s)|